Laboratory Engineer Multiplex Immunohistochemistry

About the position Kromnigon AB is a biotechnology company developing reagents and technologies for multicolour microscopy and tissue-based analysis. We are seeking a Laboratory Engineer for a three-month full-time project focused on the development, optimisation and evaluation of multiplex immunohistochemistry assays on tissue samples. The position is highly laboratory-based and requires substantial hands-on experience with histological tissue preparation and immunohistochemical staining. The successful candidate will work independently with assay execution, troubleshooting and evaluation in close collaboration with the Kromnigon research team.

• Occupation classification: SSYK 3215 – Laboratory Engineer
• Main responsibilities
• The work will primarily include:
• Development, optimisation and evaluation of multiplex immunohistochemistry (IHC) assays
• Performance of multiplex staining protocols on tissue sections
• Preparation and handling of formalin-fixed paraffin-embedded (FFPE) tissue
• Tissue sectioning and preparation of slides for histological analysis
• Deparaffinisation, antigen retrieval, blocking and immunostaining
• Optimisation of antibodies, staining conditions and assay parameters
• Evaluation of staining quality, specificity and reproducibility
• Microscopy-based assessment and documentation of experimental results
• Testing and evaluation of reagents and laboratory protocols
• Documentation of experimental procedures and results

• Required qualifications
• We are looking for a candidate with:

A relevant university degree in biomedicine, molecular biology, biotechnology, biomedical laboratory science, histology, pathology or a related life-science field Documented hands-on laboratory experience with immunohistochemistry Practical experience with multiplex immunohistochemistry and/or multiplex immunofluorescence Experience working with FFPE tissue samples Practical experience with histological techniques, including tissue sectioning, deparaffinisation and preparation of tissue sections for staining

• Experience in optimisation and troubleshooting of immunostaining protocols
• Ability to independently plan, perform, document and evaluate laboratory experiments
• Good written and spoken English

• Meritorious qualifications
• It is considered an advantage if the candidate has:
• Experience from cancer research, tumour biology or tumour pathology
• Experience analysing human tumour tissue
• Experience with multicolour fluorescence microscopy
• Experience with image-based evaluation of multiplex-stained tissue
• Previous experience developing or validating histological or immunohistochemical assays
• Experience working in a research or biotechnology environment

Employment The position is a full-time fixed-term employment for three months, based at Kromnigon AB at AstraZeneca BioVentureHub in Mölndal, Sweden. Planned starting date: as soon as possible / by agreement.